Vol. 6 No. 02 (2025)
Articles

Synergistic Antibacterial Effects of Saponified Waste Oil and Cetrimonium Chloride (Ctac) in Cement Pastes

Abdel Fattah Qaraman
Department of Health Professions, College of Intermediate Studies, Israa University, Gaza, Palestine
MAHER ELBAYOUMI
Department of Engineering and Information Technology, College of Intermediate Studies, Israa University, Gaza, Palestine

Published 2025-08-25

Keywords

  • Cementitious materials, Cetrimonium chloride (CTAC), Saponified cooking oil (SCO), Antibacterial performance, Gram-positive bacteria, Gram-negative bacteria, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Zone of inhibition.

How to Cite

[1]
A. F. Qaraman and M. . ELBAYOUMI, “Synergistic Antibacterial Effects of Saponified Waste Oil and Cetrimonium Chloride (Ctac) in Cement Pastes”, JoCEF, vol. 6, no. 02, pp. 51 - 57, Aug. 2025.

Abstract

Microbial colonization activity in cement pastes can lead to biodeterioration, compromising the structural integrity over time. This study investigates the antibacterial performance of cement pastes modified with saponified cooking oil (SCO) and cetrimonium chloride (CTAC). Different doses of CTAC and SCO were blended with cement paste on four bacterial strains: Escherichia coli and Pseudomonas aeruginosa (Gram-negative), Bacillus subtilis, and Staphylococcus aureus (Gram-positive). The results showed that B. subtilis revealed the highest sensitivity to CTAC's antibacterial activity, especially at high doses (up to 1%). This effect was enhanced by adding SCO, indicating a clear synergistic interaction. Gram-positive bacteria generally reacted more strongly to the modified cement paste comparing with Gram-negative bacteria. The results demonstrated the potential of CTAC/SCO-modified cement for use in microbial-sensitive environments such as hospitals, and food processing facilities.

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References

  1. A. Kanellopoulos, Concrete deterioration: Physical and chemical mechanisms, Handbook of Concrete Durability, Middleton Publishing Inc, Haslemere, UK, 2010, pp.1-32.?
  2. K. Knight, P. Cunningham, & S. Miller, "Optimizing supplementary cementitious material replacement to minimize the environmental impacts of concrete," Cem. Concr. Compos. 139, 2023. pp.105049.?
  3. Y. Petryna, D. Pfanner, F. Stangenberg, & W. Krätzig, "Reliability of reinforced concrete structures under fatigue," RESS, 77(3), 2002, pp.253-261.?
  4. J. Biernacki, J. Bullard, G. Sant, K. Brown, F. Glasser, S. Jones & T. Prater, "Cements in the 21st century: challenges, perspectives, and opportunities," J. Am. Ceram. Soc. 100(7), 2017, pp. 2746-2773.?
  5. C. Gaylarde, & B. Ortega-Morales, "Biodeterioration and chemical corrosion of concrete in the marine environment: too complex for prediction," Microorganisms, 11(10), 2023, pp. 2438.?
  6. N. Al-Aomary, W. Edrees, B. Al-Ofairi, & J. Thabit, "Nasal carriage of Staphylococcus aureus and its antibacterial susceptibility profiles among food handlers in Sana’a restaurants, Yemen," IUJAS, 7(2), 2024, pp. 55–70. https://doi.org/10.52865/DDHP5456
  7. J. Jenima, M. Dharshini, M. Ajin, J. Moses, K. Retnam, K. Arunachalam & R. Munoz, "A comprehensive review of titanium dioxide nanoparticles in cementitious composites," Heliyon, 10(20), 2024.?
  8. G. Gadd, M. Fomina, & F. Pinzari, "Fungal biodeterioration and preservation of cultural heritage, artwork, and historical artifacts: extremophily and adaptation," MMBR, 88(1), 2024. https://doi.org/10.1128/mmbr.00200-22
  9. Ogunsona, E. O., Muthuraj, R., Ojogbo, E., Valerio, O., & Mekonnen, T. H. (2020). Engineered nanomaterials for antimicrobial applications: A review. Applied Materials Today, 18, 100473.?
  10. L. Dyshlyuk, O. Babich, S. Ivanova, N. Vasilchenco, V. Atuchin, I. Korolkov & A. Prosekov, "Antimicrobial potential of ZnO, TiO2 and SiO2 nanoparticles in protecting building materials from biodegradation," Int. Biodeterior. Biodegrad. 146, 2020, pp.104821.?
  11. Y. Qiu, Y. Zhou, Y. Chang, X. Liang, H. Lin, X. Zhang, & Z. Luo, "The effects of ventilation, humidity, and temperature on bacterial growth and bacterial genera distribution," IJERPH, 19(22), 2022,pp.15345.?
  12. N. Falk, "Surfactants as antimicrobials: a brief overview of microbial interfacial chemistry and surfactant antimicrobial activity," J. Surfact. Deterg. 22(5), 2019, pp.1119-1127.
  13. A. P. Desbois, V. J. Smith, "Antibacterial free fatty acids: Activities, mechanisms of action, and biotechnological potential," Appl. Microbiol. Biotechnol. 85, 2010, pp.1629-1642.
  14. J. Pazmino,"Fat oil and grease formation and adhesion to concrete treated with soy methyl ester," North Carolina State University, 2023.
  15. J. Nilimaa,"Smart materials and technologies for sustainable concrete construction," Developments in the Built Environment, 15, 2023, pp.100177.
  16. A. F. Qaraman, "The efficiency of mixed surfactants as air entraining agents in cement pastes," Eur. J. Mater. Sci. 3(3), 2016, pp.12-22.
  17. A. F. Qaraman," Role of CTAC surfactant in enhancing the physical and microstructural properties of sustainable cementitious pastes," Int. J. Multidiscip. Stud. High. Educ. 2(2), 2025,pp.115-131.
  18. S. Lee, J. Kim, J. Jeong, Y. Park, G. Bai, E. Lee, " Evaluation of the broth microdilution method using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride for rapidly growing mycobacteria susceptibility testing," J. Korean. Med. Sci. 22(5), 2007, pp.784-790.
  19. P. Sabaeifard, A. Abdi-Ali, M. R. Soudi, R. Dinarvand,"Optimization of tetrazolium salt assay for Pseudomonas aeruginosa biofilm using microtiter plate method," J. Microbiol Methods. 105, 2014, pp.134-140.
  20. D. Otzen, J. Pedersen, H. Rasmussen, J. Pedersen," How do surfactants unfold and refold proteins?" Adv. Colloid. Interface. Sci. 308, 2022, pp.102754.
  21. C. Gloxhuber, K. Klunstler, "Anionic surfactants: biochemistry, toxicology, dermatology" Vol. 43. CRC Press, 1992.
  22. P. Sharma, R. Vaiwala, S. Parthasarathi, N. Patil, A. Verma, M. Waskar," Interactions of surfactants with the bacterial cell wall and inner membrane: revealing the link between aggregation and antimicrobial activity," Langmuir. 38(50), 2022, pp.15714-15728.
  23. S. Negi, Surfactants as antimicrobial nanocoatings for medical devices and implants. In: Next-Generation Antimicrobial Nanocoatings for Medical Devices and Implants. Woodhead Publishing; 2024, pp.181-204.
  24. S. Kelkar, K. Bhojwani, E. Kamble, Biosurfactants as antimicrobial agents. In: Microbial Surfactants in Pharmaceuticals and Cosmetics. CRC Press, 2025, pp.256-273.
  25. S. Buffet-Bataillon, P. Tattevin, M. Bonnaure-Mallet, A. Jolivet-Gougeon," Emergence of resistance to antibacterial agents: the role of quaternary ammonium compounds—a critical review," Int. J. Antimicrob. Agents. 39(5), 2012, pp.381-389.
  26. Y. Chen, F. Qiao, Y. Fan, Y. Han, Y. Wang," Interactions of cationic/anionic mixed surfactant aggregates with phospholipid vesicles and their skin penetration ability," Langmuir. 33(11) 2017, pp.2760-2769.
  27. C. Wang, X. L. Cao, L. L. Guo, Z. C. Xu, L. Zhang, Q. T. Gong, " Effect of molecular structure of catanionic surfactant mixtures on their interfacial properties," Colloids. Surf. A. Physicochem. Eng. Asp. 509, 2016, pp.601-612.
  28. R. Wang, H. Yan, W. Ma, Y. Li," Complex formation between cationic gemini surfactant and sodium carboxymethylcellulose in the absence and presence of organic salt," Colloids. Surf. A. Physicochem. Eng. Asp. 509, 2016, pp.293-300.
  29. D. Varade, D. Carriere, L. R. Arriaga, A. L. Fameau, E. Rio, D. Langevin, "On the origin of the stability of foams made from catanionic surfactant mixtures," Soft. Matter. 7(14), 2011, pp.6557-6570.
  30. E. W. Kaler, A. K. Murthy, B. E. Rodriguez, J. A. Zasadzinski," Spontaneous vesicle formation in aqueous mixtures of single-tailed surfactants," Science. 245(4924), 1989, pp.1371-1374.
  31. E. Akpinar, N. Uygur, G. Topcu, O. Lavrentovich, A. Neto, "Gemini surfactant behavior of conventional surfactant dodecyltrimethylammonium bromide with anionic azo dye Sunset Yellow in aqueous solutions," J. Mol. Liq. 360 2022, pp.119556.
  32. S Hou, J Li, Y Wang, Y Jiang, Z Wang, T Geng. Synergistic effects of Gemini cationic surfactants with multiple quaternary ammonium groups and anionic surfactants with long EO chains in the mixed systems. J Mol Liq. 376, 2023, pp.121427.
  33. K. Xu, J. Yang, H. He, J. Wei, Y. Zhu," Influences of additives on the rheological properties of cement composites: A review of material impacts," Materials, 18(8), 2025,pp.1753.